A monoclonal antibody specific for a 40 kDa polypeptide recognizes pathogenic strains of Entamoeba histolytica

In vitro interaction of Entamoeba hystolytica trophozoites with collagen induces the intracellular formation and release of electron-dense granules (EDGs). We determined that four polypeptides in EDGs total antigen by SDS-PAGE were absent in trophozoite extracts. A monoclonal antibody raised against these EDGs recognized a polypeptide of 40 kDa specific for the pathogenic strains of E. histolytica. In addition, this mAb recognized a 96 kDa peptide masked by carbohydrates

Electrophysiology and immunity of the mucosa in intestinal infections

We present here some of the major concepts and approach to study the electrophysiology of the intestinal mucosa, and review the pathophysiology of intestinal infections caused by enteropathogenic bacteria, protozoa - specially our own work on experimental amebiasis using intestinal preparations mounted in the Ussing chamber - and nematodes, and finally discuss briefly the immunophysiology of the intestinal mucosa

Detection of entamoeba histolytica isoenzyme profiles in Egyptians having amoebiasis

Microscopic examination and culture in Robinson's medium of 179 fresh stool samples revealed the presence of E. histolytica in 94 cases [19 with acute amoebic dysentery, 45 with chronic amoebic dysentery and 30 asymptomatic carriers], other protozoa in 50 samples and 35 samples were found to be free from parasitic infestations. Using the mobility patterns of L-malate: NADP [+] oxidoreductase [ME] on polyacrylamide gel isoelectric focusing, all E. histolytica positively cultured isolates gave a characteristic ME band 21 mm above the insertion point. Distinctly different patterns for other protozoa were detected. Pathogenic strains of E. histolytica gave a distinctive fast moving phosphoglucomutase [PGM] beta band [10 mm above the insertion point]. Pathogenic strains were identified in 39 of 94 [41.5 percent] isolates and these strains were found to be strongly associated with dysentery and trophozoites containing ingested red blood corpuscles

Inmunoprofilaxia de la amibiasis / Inmunoprophilaxis of the amoebiaisis

La amibiasis es la infección producida por el protozoario parásito Entamoeba histolytica. A pesar de que el término amibiasis incluye a todos los casos humanos de infección producidos por este microorganismo, sólo una parte de los individuos infectados presentan síntomas imputables a la penetración de las amibas en los tejidos. A esta entidad nosológica se le conoce como amibiasis invasora y al grupo de personas infectadas asintomáticamente se les denomina portadores de E. histolytica y presentan amibiasis luminal. Los estudios sobre inmunidad protectora antiamibiana se encuentran todavía en etapa experimental; sin embargo, en animales de laboratorio los resultados obtenidos han sido en general satisfactorios. Los primeros intentos de inducción de protección antiamibiana, llevados a cabo por diferentes grupos, tuvieron éxito en general. Sin embargo, hay una gran falta de homogeneridad en las condiciones utilizadas por cada grupo de investigadores, y principalmente han consistido en el uso de diferentes dosis de antígenos, en los métodos de caracterización de las cepas amibianas utilizadas, las cantidades de inóculo administradas, las vías de inmunización y los modelos animales en que se aplicaron

Biochemical evaluation in entamoeba histolytica infected persons in Sharkia governorate

Serum total, direct and indirect bilirubin, serum asparate aminotransferase [SGOT], serum alanine aminotransferase [SGPT], total protein, albumin, globulin, blood urea and creatinine were estimated in twenty five Entamoeba histolytica cyst passers, five trophozoite passers and five healthy control persons. It was found that in trophozoite passers there was an increase in total bilirubin than normal and no changes in indirect bilirubin. However, direct bilirubin, serum alanine aminotransferase [SGPT], serum aspartate aminotransferase [SGOT], total protein, albumin, globulin, urea and creatinine were decreased. While in cysts passers all above parameters were increased except blood urea was decreased. There was direct relationship between the intensity of infection and tested parameters in both cyst and trophozoite passers